Overpacking the autoclave may result in what issue?

Overpacking the autoclave can lead to significant issues with the sterilization process, primarily due to the diminished ability of steam to penetrate the packed materials effectively. When an autoclave is overloaded, the steam cannot circulate freely, which is essential for reaching all surfaces of the instruments and materials inside. This hinders the sterilization cycle, as steam must contact every surface to ensure that all pathogens are killed.

Additionally, adequate air removal and steam penetration are crucial for effective sterilization. An overpacked autoclave compromises these principles, reducing the overall efficacy of the sterilization process. Therefore, the correct response highlights a critical aspect of maintaining proper sterilization protocols in a dental or medical setting.